Helios gene gun manual

Particle bombardment protocol was carried out using the Helios™ Gene Gun device (BioRad) and the manufacturer's instruction manual. Helium pressure (50, . The Helios gene gun is a convenient handheld device that provides rapid and direct transfection into a range of targets in vivo. The unit uses an adjustable low-pressure helium pulse to sweep DNA-, RNA-, or biomaterial-coated gold microcarriers from the inner wall of a small plastic cartridge directly into target cells. 1. General Gene Gun Safety- Due to the velocity of the expelled microparticles, the gene gun should always be aimed in a direction away from people. Whenever the gene gun is in use, signs should be posted outside of the room to warn others and doors should be locked. The gene gun is to be secured in a locked cabinet when not in use. 2.

Choiceofgenegunsystem. The hand-held Bio-Rad Helios Gene Gun is the system of choice. The ability to vary the pressure for bombardment down to levels lower than those obtained with the standard high-pressure fixed gene gun setups, such as the Biolistic PDSHe Particle Delivery System, so as to avoid extensive. 1. General Gene Gun Safety- Due to the velocity of the expelled microparticles, the gene gun should always be aimed in a direction away from people. Whenever the gene gun is in use, signs should be posted outside of the room to warn others and doors should be locked. The gene gun is to be secured in a locked cabinet when not in use. 2. Bio-rad Helios Gene Gun System Instruction Manual Instruction manual (60 pages) MedKlinn Autoplus Operating Manual Operating manual (7 pages) Coltene SciCan STATCLAVE G4 Operator's Manual Operator's manual (64 pages) Sutter Instrument P Operation Manual Operation manual (88 pages).

Particle bombardment protocol was carried out using the Helios™ Gene Gun device (BioRad) and the manufacturer's instruction manual. Helium pressure (50, , and psi) and gold particle size. 1. General Gene Gun Safety- Due to the velocity of the expelled microparticles, the gene gun should always be aimed in a direction away from people. Whenever the gene gun is in use, signs should be posted outside of the room to warn others and doors should be locked. The gene gun is to be secured in a locked cabinet when not in use. 2. The parts used in manufacturing the Helios Gene Gun have been chosen because they are designed to work at the pressures required for oper-ation and have a wide safety margin. General safety principles are indicated below. Specific safety recommendations are indicated in the appropriate sections throughout the manual. Helios Gene Gun Safety.